FPA: Fiber Photometry Analyzer.

Analyze Fiber Photometry Data with Ease- No Coding Required.

Experts in Fiber Photometry.

At Metofico we are experts in Fiber Photometry, a technique which measures population-level calcium dynamics in awake behaving animals.

We not only provide consulting in this technique (included in yearly license) but a state-of-the-art analyzer for Fiber Photometry data.

This module makes analysis of Fiber Photometry data accessible to all, regardless of coding ability or expertise in signal processing, without which analysis is typically unachievable.

FPA: Benefits.

Efficiency

FPA allows for fast processing of vast data sets without writing a single line of code, thus accelerating scientific output.

Reliability

By leveraging FPA, you ensure high-quality data processing and analysis powered by the latest published algorithms.

Repeatability

FPA ensures precise, repeatable analysis—same data, same parameters, same results—every time, regardless of skill level.

Learn More:

Data Input

  • Batch import supporting several file formats

  • Up to 4 light channels per animal

Filtering & Smoothing

  • Effortlessly eliminate large artefacts from your data

  • Enhance data clarity using digital filters

Animal Manager

  • Conveniently segment your data

  • Define events as single points or as time-bounded events for precise analysis  

Event Manager

  • Organize subjects into groups

  • perform individual/group analyses          

Normalization & Analysis

  • Several normalization & analysis pipelines to suit a variety of experimental designs

Plotting Features

  • Built-in plotting features to quickly explore your data including a variety of line plots, bar plots and heat maps

Associated Publications.

Journal of Neuroscience 2023
M2 Cortex Circuitry and Sensory-Induced Behavioral Alterations
Conde-Berriozabal, S., García-Gilabert, L., García-García, E., Sitjà-Roqueta, L., López-Gil, X., Muñoz-Moreno, E., Boutagouga Boudjadja, M., Soria, G., Rodríguez, M.J., Alberch, J., Masana, M. Publication Link
Current Biology 2022
Hypothalamic AgRP neurons exert top-down control
Boutagouga Boudjadja, M., Culotta, I., De Paula, G.C., Harno, E., Hunter, J., Cavalcanti-de-Albuquerque, J.P., Luckman, S.M., Hepworth, M., White, A., Aviello, G., D’Agostino, G. Publication Link
Cell Metabolism 2021
OPA1 in POMC neurons couples Ca2+ homeostasis
Gómez-Valadés, A.G., Pozo, M., Varela, L., Boudjadja, M.B., Ramírez, S., Chivite, I., Eyre, E., Haddad-Tóvolli, R., Obri, A., Milà-Guasch, M., Altirriba, J., Schneeberger, M., Imbernón, M., Claret, M. Publication Link

Frequently Asked Questions (FAQ’s).

  • Fiber photometry is a powerful technique for monitoring population-level calcium dynamics in awake, behaving animals. It involves the implantation of a fiber-optic cannula, which delivers excitation light to neurons that express genetically encoded calcium indicators and collects fluorescence signals that reflect calcium fluctuations. These calcium transients serve as indirect measures of neuronal activity, allowing researchers to investigate correlations between neural dynamics and behavioral states.

  • Our modules are fully no-code, meaning you can use them from start to finish without writing a single line of code. This is crucial because programming skills are often a barrier to efficient and advanced data analysis. By eliminating this requirement, we ensure that high-quality data analysis is accessible to all researchers, regardless of coding experience.

  • Although open-source software is technically free, the significant time and effort required for implementation and customization can lead to substantial hidden costs. Moreover, once deployed, these tools often suffer from poor interface design and a lack of critical features, which hampers usability. In contrast, FPA provides a robust no-code tool accompanied by expert guidance.

  • The software was initially designed to be compatible with doric hardware systems, which is what ourselves and our collaborators have primarily used. However, we can easily adapt the software to work with other systems by modifying the data extraction and organization process.

  • FPA is designed to accept Excel file inputs, as this is the most common output format from fiber photometry hardware systems. However, we can modify our software if other data input formats are required.


  • The inbuilt software systems often lack the usability and features needed to effectively process and analyze fiber photometry data sets. This is based on both our experience and that of our collaborators.

  • At Metofico we constantly update our software based on customer feedback and market demand, keeping our solutions up-to-date with advancements in the field.

Get In Touch.

To find out more about our products and services please contact us at info@metofico.com, via social media or fill out the form.

We offer free demos and trials of our software, allowing you to explore how our solutions can enhance your specific research or operational goals.